La determinación de proteínas es uno de los análisis clave que se realizan en la industria alimentaria. Las muestras requieren digestión con ácido sulfúrico para convertir el nitrógeno en sulfato de amonio. Después de la conversión a amoníaco mediante alcalinización con hidróxido de sodio, la muestra se destila en un receptor de ácido sulfúrico mediante destilación al vapor, seguido de una retrovaloración con solución de hidróxido de sodio. El contenido de nitrógeno se multiplica por un factor específico de la muestra (6,25 para pasta) para obtener el contenido de proteína.
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